Scott Nichols Writer - Johnston, IA
Name variants: Scotty Nichols
Professions & Specialties
Promoter • Host
Experience & Education
| United States Patent | Inventor | Appl. No. | Assignee |
|---|---|---|---|
|
#6399859 Jun 4, 2002 Filed: Dec 10, 1997 |
Scott E. Nichols (Johnston, IA) Kanwarpal S. Dhugga (Johnston, IA) George W. Singletary (Ankeny, IA) Court A. Saunders (Clive, IA) Derrick Witcher (Fishers, IN) Wesley B. Bruce (Urbandale, IA) Lynne E. Sims (Polk City, IA) Guihua Lu (Urbandale, IA) | 08/987367 |
Pioneer Hi-Bred International, Inc. (Des Moines, IA) |
| International Classification: | C12N 1582 | 800298, 800295, 8003001, 8003172, 8003201, 8003202, 8003203, 435412, 435417, 435418, 435419, 4352551, 435 257, 4353201, 435410, 536 231, 536 232, 536 236 |
Patent:
Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof
Abstract:
Isolated nucleic acid molecules are provided that encode maize UDPGdH variant UDPGdH, and mutant UDPGdH proteins. These nucleic acid molecules can be used to produce transgenic plants having altered quality or quantity of starch. Also provided are vectors capable of expressing such nucleic acid molecules, host cells containing, such vectors, and polypeptides encoded by such nucleic acids.
Claim:
We claim: 1. An isolated nucleic acid molecule comprising a polynucleotide encoding a UDP-glucose dehydrogenase (UDPGdH) polypeptide comprising an amino acid sequence of either SEQ ID NO:2 or SEQ ID NO:4. 2. The isolated nucleic acid molecule of claim 1 wherein the polypeptide has an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4. 3. An isolated nucleic acid molecule encoding a UDPGDH polypeptide having at least 98% sequence identity to SEQ ID NOS: 1 or 3 or a full complement thereof. 4. The isolated nucleic acid molecule of claim 3 having the nucleotide sequence of either SEQ ID NO:1 or SEQ ID NO:3 or a full complement thereof. 5. An expression vector comprising a promoter that is operably linked with the nucleic acid molecule of claim 3. 6. A host cell comprising the expression vector of claim 5, wherein said host cell is selected from the group consisting of a bacterium, yeast cell, insect cell, and plant cell. 7. The host cell of claim 6, wherein said host cell is a plant cell selected from the group consisting of maize cell, sorghum cell, wheat cell, rice cell, barley cell, oat cell, and potato cell. 8.
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